Anti-KSR1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IVT5

Core Product Specifications and Parameters

Biological Background: KSR1 Function and Localization

• KSR1 (Kinase suppressor of Ras 1) is a scaffold protein and putative serine/threonine-protein kinase that plays a central role in the MAPK signaling cascade.
• It forms part of a multiprotein signaling complex that promotes phosphorylation of Raf family members and activation of downstream MAP kinases.
• Acts as a MAP2K1/MEK1- and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MEK, it dimerizes with BRAF and stimulates BRAF-mediated MEK phosphorylation (PubMed:29433126). Related references: PMID:29433126
• Promotes activation of MAPK1/ERK2 and MAPK3/ERK1 in response to EGF and cAMP stimulation.
• Subcellular localization includes cytoplasm, cell membrane, ruffle membrane, and endoplasmic reticulum membrane, consistent with its dynamic signal transduction roles.
• Contains a zinc-finger domain and exhibits ATP-binding activity; however, its intrinsic kinase activity remains disputed and may be context-dependent.
• Alternative splicing generates multiple isoforms, and the protein is regulated by phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen (aa 320–420) lies outside the kinase domain; sequence homology with mouse and rat KSR1 supports cross-reactivity, but alignment should be verified for the exact epitope region.
• For Western blot, a band near 102 kDa is expected; optimize lysis and loading conditions using positive control cell lines (e.g., HeLa, NIH/3T3).
• In immunofluorescence/ICC, test both methanol and formaldehyde fixation, as KSR1 localizes to membrane ruffles and the endoplasmic reticulum; permeabilization with Triton X-100 or digitonin may be necessary.
• For ELISA, consider pairing with a detection antibody against a non-overlapping epitope after validating binding affinity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a complete portfolio of IVD raw materials and tailored sourcing support. This anti-KSR1 polyclonal antibody serves MAPK pathway research and assay development. Beyond this product, we supply validated antibody pairs, optimized monoclonal/ polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to accelerate your projects from concept to clinic.