Anti-KRT81 Rabbit Polyclonal Antibody for WB, ELISA - Q14533

Core Product Specifications and Parameters

Biological Background: KRT81 Function and Localization

• KRT81, also known as Keratin 81 (aliases: HB1, K81, KRTHB1, MLN137), is a type II hair keratin that belongs to the intermediate filament family, characterized by coiled-coil domains and potential for isopeptide bond and ubiquitin-like conjugation modifications.
• As a structural component, KRT81 forms obligate heterodimers with type I hair keratins, which assemble into keratin intermediate filaments essential for the mechanical strength and integrity of the hair shaft. Related references: PMID:10504448, PMID:9402962
• It is abundantly expressed in the differentiating cortex of growing (anagen) hair follicles, specifically in keratinocytes of the hair cortex, and is absent from the inner root sheath and medulla. Related references: PMID:11445569
• KRT81 is also detected in malignant lymph node tissue associated with breast carcinoma, and Uniprot keywords associate it with disease variants, suggesting roles beyond hair biology.
• The protein has a predicted molecular weight of approximately 55kDa and is encoded by the KRT81 gene (also cited as KRTHB1 or MLN137).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 350–450 of human KRT81 (NP_002272.2). This C-terminal region was selected to potentially avoid cross-reactivity with other keratins; however, sequence homology should be verified against related type II keratins.
• For Western blotting, expect a single band at approximately 55kDa in human hair follicle lysates or appropriate tissue extracts; using reducing conditions may aid in solubilizing keratin filaments. Validate with mouse samples as cross-reactivity is indicated; cross-reactivity with other species should be determined empirically.
• In ELISA, the antibody can be employed for direct antigen detection; consider peptide competition assays to confirm specificity if needed.
• Due to the insolubility of keratins, include a denaturing and reducing sample buffer during lysis and consider sonication to release KRT81 from the intermediate filament network.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, offering one-stop access to validated antibodies, ancillary reagents, and custom production services. This anti-KRT81 rabbit polyclonal antibody is part of our extensive catalog for hair keratin and intermediate filament research. Whether you need optimized antibody pairs, bulk monoclonal/polyclonal antibodies, or rare target sourcing, CamelBio bridges the gap from initial concept to clinical application, supporting diagnostic manufacturers and research laboratories worldwide.