Anti-KRT80 Rabbit Polyclonal Antibody for IF/ICC, IF-P, ELISA - Q6KB66

Core Product Specifications and Parameters

Biological Background: KRT80 Function and Localization

• Keratin, type II cytoskeletal 80 (KRT80), also known as cytokeratin-80 (CK-80), keratin-80 (K80), and type-II keratin Kb20, is a member of the keratin family of intermediate filament proteins.
• Encoded by the KRT80 (KB20) gene, this protein exhibits alternative splicing, producing multiple isoforms that may have distinct functional roles.
• KRT80 is a type II keratin that forms coiled-coil dimers essential for the assembly of intermediate filaments, providing structural support to cells.
• Subcellularly, KRT80 localizes to the intermediate filament network, contributing to cytoskeletal integrity and cellular mechanics.
• Phosphorylation of KRT80 (as indicated by proteomics data) may regulate filament dynamics and interactions with other cellular components.
• Tissue expression analysis reveals weak expression in the tongue, with no detected expression in skin or most other tissues and organs, suggesting a specialized role in oral epithelia.

Experimental Guidance and Technical Tips

• For immunofluorescence applications (IF/ICC and IF-P), consider optimizing fixation and permeabilization protocols to preserve the intermediate filament network. Methanol or paraformaldehyde fixation followed by mild detergent permeabilization may be suitable starting points.
• Given the weak tissue expression of KRT80, pre-validation in a relevant sample system (such as tongue tissue sections or cell lines known to express keratin 80) is recommended to confirm specific staining.
• In ELISA applications, consider using the immunogen sequence (aa 242–452) as a control antigen to establish assay sensitivity and specificity, and perform antibody titration to determine optimal working concentrations.
• As cross-reactivity with mouse and rat is reported, the antibody can be explored in rodent models; however, sequence alignment of the target region across species is advised to predict reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for diagnostic manufacturers and research labs to source high-quality IVD raw materials. From validated antibody pairs and optimized polyclonal antibodies like this anti-KRT80 pAb to bulk ancillary reagents and rare-target sourcing, we bridge the gap from concept to clinic. Accelerate your intermediate filament and epithelial biology projects with reliable, customizable raw-material solutions.