Anti-KRT3 Polyclonal Antibody for WB, IF/ICC, ELISA - P12035

Core Product Specifications and Parameters

Biological Background: KRT3 Function and Localization

• Keratin, type II cytoskeletal 3 (KRT3), also known as CK-3 or 65 kDa cytokeratin, is a member of the keratin intermediate filament family.
• It is specifically expressed in the corneal epithelium, where it forms heteropolymeric filaments with the type I keratin KRT12 to provide mechanical strength and structural integrity.
• Together with KRT12, KRT3 constitutes the major keratin pair of the corneal epithelium, essential for maintaining corneal transparency and resilience.
• Mutations in KRT3 are associated with Meesmann corneal dystrophy, a disease characterized by corneal epithelial microcysts.
• The protein contains a central coiled-coil rod domain typical of keratins, facilitating filament assembly.
• Post-translational modifications include phosphorylation and methylation, which may regulate filament dynamics.
• KRT3 is classified under the type II keratin subfamily and is a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 230-390, which lies within the central rod domain of KRT3. This region is likely to be conserved, so verify specificity for KRT3 versus other type II keratins if needed.
• For Western blotting (WB), a band around 64 kDa is expected in human corneal epithelial lysates. Consider using positive controls such as corneal epithelium cell lines or tissue extracts.
• For immunofluorescence (IF/ICC), use corneal epithelial cells and ensure appropriate fixation/permeabilization protocols to visualize the cytoplasmic intermediate filament network.
• In ELISA, the antibody can be employed to detect recombinant or native KRT3; optimize coating and detection conditions.
• Since cross-reactivity is listed only for human, validate in other species if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in supplying high-quality IVD raw materials, offering a comprehensive range of antibodies, including validated monoclonal and polyclonal options for research and diagnostic development. Our KRT3 polyclonal antibody supports studies into corneal epithelial biology and corneal dystrophies, and we can assist with sourcing rare target reagents, bulk ancillary materials, and technical consultation to accelerate your project from concept to clinic.