Anti-KRT10 Rabbit Polyclonal Antibody for WB and ELISA - P13645

Core Product Specifications and Parameters

Biological Background: KRT10 Function and Localization

• Keratin 10 (K10/CK-10) is a type I cytoskeletal keratin that forms intermediate filaments, providing mechanical support to suprabasal keratinocytes.
• Obligate heteropolymer partner for type II keratins KRT1 and KRT2, critical for terminal differentiation and epidermal barrier function.
• Acts as a receptor for Staphylococcus aureus clfB on nasal epithelial cells, facilitating nasal colonization.
• Mediates Streptococcus pneumoniae adherence to lung epithelia via PsrP binding, influencing bacterial infection.
• Localized intracellularly in the cytoplasm, on the cell surface, and secreted extracellularly.
• Expressed throughout suprabasal epidermal layers (including stratum corneum) and on the surface of lung cell lines and desquamated nasal epithelial cells.
  Related references: PMID:19627498 PMID:12427098
• Post-translational modifications include phosphorylation and disulfide bond formation; mutations are linked to ichthyosis.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody targets a synthetic peptide within amino acids 350–460 of human KRT10 (NP_000412.3). For Western blot, expect a band near 59 kDa; use appropriate positive controls such as human keratinocyte lysates.
• For ELISA, optimize coating conditions and consider using the immunogen peptide as a positive control for direct or competitive ELISA formats.
• Although reported to be human-specific, cross-reactivity with KRT10 from other species has not been tested; validate in the relevant sample system.
• Polyclonal antibodies may exhibit lot-to-lot variability; confirm performance with reference standards and include negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing platform for IVD raw materials, addressing the needs of diagnostic manufacturers and researchers from early development to clinical application. Our portfolio includes validated antibodies (polyclonal and monoclonal pairs), recombinant proteins, and ancillary reagents. For studies on intermediate filaments, keratinocyte biology, or bacterial adherence, we can scale supply of anti-KRT10 antibodies and related targets, while our sourcing network readily accommodates custom and rare-target requests.