Anti-KREMEN1 Polyclonal Antibody for WB, ELISA - Q96MU8

Core Product Specifications and Parameters

Biological Background: KREMEN1 Function and Localization

• KREMEN1 (Kremen protein 1), also known as Dickkopf receptor, kringle domain-containing transmembrane protein 1, or kringle-containing protein marking the eye and the nose, is a single-pass type I transmembrane receptor.
• It functions as a receptor for Dickkopf (DKK) proteins, cooperating with DKK1/2 to inhibit Wnt/β-catenin signaling by promoting the endocytosis of LRP5/6 co-receptors.
• In the absence of DKK ligands, KREMEN1 can potentiate Wnt/β-catenin signaling by maintaining LRP5 or LRP6 at the cell membrane.
• KREMEN1 plays critical roles in development: it attenuates Wnt signaling in the developing limb to ensure normal limb patterning, and negatively regulates bone formation.
• The protein contains a kringle domain and undergoes post-translational modifications including glycosylation and disulfide bond formation.
• KREMEN1 is localized to the cell membrane.
• Alternative splicing produces multiple isoforms.
• It has a Wnt-independent apoptotic activity, which is inhibited by DKK1 binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 180-380 of human KREMEN1 (NP_001034659.2), which lies within the extracellular region, likely targeting the kringle domain. Consider verifying reactivity with relevant isoforms in your sample system.
• For Western blot, a band around 52 kDa is expected; optimize loading and antibody dilution for your specific cell or tissue lysates.
• This polyclonal antibody is suitable for ELISA; consider pairing with a matched detection antibody for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with a one-stop sourcing bridge for IVD raw materials, technical services, and consulting from concept to clinic. For Wnt signaling and developmental research, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist with rare target raw-material sourcing. Contact us to support your KREMEN1-related assay development and procurement needs.