Anti-KRAS+HRAS+NRAS Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - P01111/P01112/P01116

Core Product Specifications and Parameters

Biological Background: KRAS+HRAS+NRAS Function and Localization

• NRAS functions as a signal transducer in the RAS-MAPK signaling pathway, regulating cell proliferation and survival through cyclic binding of GDP/GTP and intrinsic GTPase activity (PubMed:30712867).
  Related references: PMID:30712867
• KRAS, HRAS, and NRAS share high sequence similarity, particularly in the N-terminal domain (aa 1-100), which is the immunogen region, enabling this antibody to detect all three isoforms.
• Oncogenic mutations in NRAS (commonly at codons 12, 13, and 61) are frequent in human cancers, and the antibody recognizes both wild-type and mutant forms, making it valuable for oncology research.
• NRAS localizes primarily to the plasma membrane and the Golgi apparatus, where it mediates signaling events at these sites.
• Post-translational modifications, such as prenylation and palmitoylation, are critical for membrane targeting and biological activity of NRAS.
• The E3 ubiquitin ligase LZTR1 mediates ubiquitination of NRAS, leading to proteasomal degradation and serving as a regulatory mechanism (PubMed:40934300).
  Related references: PMID:40934300

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1-100 of human Ras (P01111), a conserved region across KRAS, HRAS, and NRAS. Consider validating specificity using isoform-specific knockdown or knockout cell lines if needed.
• For western blotting (WB), a band around 21 kDa is expected under reducing conditions. Include appropriate loading controls and consider using RIPA lysis buffer for effective extraction of membrane-associated Ras.
• In immunofluorescence (IF/ICC), Ras proteins are typically observed at the plasma membrane and Golgi; permeabilization is required. Nuclear staining is not expected.
• For immunoprecipitation (IP), use optimized lysis buffers containing non-ionic detergents and protease/phosphatase inhibitors to preserve protein-protein interactions. Pre-clearing and antibody titration may improve specificity.
• ELISA performance should be evaluated with recombinant Ras protein or cell lysates; the detection limit will depend on the assay format and detection system used.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing platform, supplying this pan-Ras monoclonal antibody to diagnostic developers and researchers studying the RAS signaling axis. Our comprehensive catalog includes validated antibody pairs, bulk ancillary reagents, and customized procurement for rare targets, ensuring a seamless pipeline from research to clinical assay development. Whether you need high-quality antibodies for biomarker discovery or large-scale production, CamelBio provides reliable, one-stop access to accelerate your projects.