Anti-KRAS+HRAS+NRAS Monoclonal Antibody for WB, IF/ICC, ELISA - P01111/P01112/P01116

Core Product Specifications and Parameters

Biological Background: KRAS+HRAS+NRAS Function and Localization

• NRAS is a small GTPase that acts as a signal transducer in the Ras-MAPK signaling pathway, regulating cell proliferation and survival.
  Related references: PMID:30712867
• NRAS binds GDP/GTP and possesses intrinsic GTPase activity, cycling between active GTP-bound and inactive GDP-bound states.
  Related references: PMID:30712867
• Recognized by LZTR1, an adaptor of the BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex, which mediates NRAS ubiquitination.
  Related references: PMID:40934300
• Localizes to the cell membrane and Golgi apparatus membrane, consistent with its roles in signal transduction from cell surface receptors and intracellular trafficking.
• Belongs to the Ras oncogene family, which includes the closely related KRAS and HRAS proteins; all are frequently mutated in human cancers.
• Post-translational modifications include palmitoylation, prenylation, phosphorylation, acetylation, and ubiquitination, which regulate its membrane association and activity.
• Serves as a critical proto-oncogene; activating mutations in NRAS are associated with various malignancies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–188) of human NRAS, a domain conserved across KRAS, HRAS, and NRAS; this conservation likely accounts for the antibody’s pan‑RAS reactivity observed in the cross‑reactivity data.
• For western blotting, NRAS migrates at a predicted molecular weight of ~21 kDa; include appropriate molecular weight markers and validate the band pattern in your specific sample system.
• In immunofluorescence, expect cell membrane and Golgi staining; optimize fixation and permeabilization conditions and include appropriate negative controls to confirm specificity.
• For ELISA, consider pairing this antibody with a suitable detection system; perform a titration series to determine the optimal working concentration for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated sourcing partner for IVD manufacturers and research laboratories, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate assay development. In the Ras signaling and oncology biomarker research field, CamelBio offers tailored procurement solutions and technical consultation, helping you transition from concept to clinical application with confidence. For rare-target raw materials or custom needs, our global network ensures reliable access to high-quality reagents.