Anti-KPNB1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q14974

Core Product Specifications and Parameters

Biological Background: Importin subunit beta-1 (KPNB1) Function and Localization

• Nuclear import receptor: KPNB1 functions as a nuclear import receptor either by associating with importin-alpha adaptors that bind classical nuclear localization signals (NLS) or by directly recognizing NLS sequences on cargo proteins. It mediates the docking of the transport complex to the nuclear pore complex (NPC) through interactions with FG-repeat nucleoporins, followed by Ran-dependent translocation (PubMed:10228156, PubMed:11682607, PubMed:11891849). Related references: PMID:10228156, PMID:11682607, PMID:11891849
• Cargo import: It imports ribosomal proteins RPL23A, RPS7, and RPL5 autonomously, and in complex with IPO7, it imports H1 histone. In vitro, it also mediates nuclear import of core histones H2A, H2B, H3, and H4 (PubMed:11682607, PubMed:9687515, PubMed:10228156). Related references: PMID:11682607, PMID:9687515, PMID:10228156
• Additional cargos: KPNB1 mediates the nuclear import of transcription factors and kinases, including MRTFA, SNAI1, and PRKCI, thereby regulating gene expression and cell signaling (PubMed:11891849, PubMed:19386897, PubMed:20818336). Related references: PMID:11891849, PMID:19386897, PMID:20818336
• Subcellular localization: It is localized in the cytoplasm and at the nuclear envelope, consistent with its role in shuttling cargo proteins through the nuclear pore complex.
• Post-translational modifications: KPNB1 undergoes acetylation, ADP-ribosylation, and phosphorylation, which may modulate its transport activity and interactions with cargo or the nuclear pore.
• Alternative splicing: The KPNB1 gene produces multiple isoforms through alternative splicing, potentially expanding its functional repertoire.
• Host-virus interaction: It plays a role in HIV-1 infection by binding to and mediating the nuclear import of the viral Rev protein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 300–400 of human KPNB1. This region is highly conserved among human, mouse, and rat orthologs, likely contributing to the antibody's cross-reactivity.
• For Western blot, the predicted molecular weight is approximately 97 kDa. Adequate separation in mid-percentage SDS-PAGE gels (e.g., 8–10%) is recommended to resolve the target band.
• In immunohistochemistry (IHC-P), antigen retrieval and antibody dilution should be empirically optimized for each tissue type and fixation method.
• As a monoclonal antibody, it provides high specificity and lot-to-lot consistency, making it suitable for quantitative assays and reproducible experiments.
• While ELISA application is listed, the appropriate antigen coating and detection conditions may require individual optimization for best performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated sourcing partner for IVD raw materials, supporting diagnostic developers and researchers from concept to clinic. This KPNB1 rabbit monoclonal antibody is part of our extensive portfolio for nuclear transport and cell signaling research. We offer validated antibody pairs, bulk reagents, and customized procurement solutions to streamline your assay development and manufacturing workflows.