Anti-KPNA6 Rabbit Polyclonal Antibody for WB, ELISA - O60684

Core Product Specifications and Parameters

Biological Background: KPNA6 Function and Localization

• KPNA6 (importin subunit alpha-7), also known as karyopherin subunit alpha-6, is a member of the importin alpha family that functions as an adapter for nuclear protein import.
• It recognizes and binds to nuclear localization signals (NLSs) on cargo proteins, accommodating both simple and bipartite NLS motifs.
• KPNA6 complexes with importin beta (KPNB1) to dock at the nuclear pore complex (NPC) via nucleoporin FxFG repeats, facilitating translocation into the nucleus.
• The import cycle is Ran GTPase-dependent, with directionality governed by the asymmetric distribution of GTP- and GDP-bound Ran across the nuclear envelope.
• Post-translational modifications of KPNA6 include acetylation and phosphorylation, which may regulate its function.
• The protein is widely expressed in human tissues and is implicated in host-virus interactions, potentially mediating viral nuclear entry.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 277-536, covering the C-terminal region rich in Armadillo (ARM) repeats critical for NLS binding. This design may reduce cross-reactivity with other importin alpha isoforms but validation in your specific system is recommended.
• For Western blotting, the predicted molecular weight of KPNA6 is approximately 60 kDa. Use appropriate lysis buffers (e.g., RIPA) and consider nuclear-enriched fractions for enhanced detection.
• ELISA protocols may be optimized using purified recombinant KPNA6 or lysates from relevant cell lines as coating antigens; include controls to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material partner, providing validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents to diagnostic manufacturers and research institutes. This anti-KPNA6 polyclonal antibody supports nuclear transport studies, and we can source additional KPNA family members or rare-target raw materials to accelerate your assay development from concept to clinic.