Anti-KPNA6 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O60684

Core Product Specifications and Parameters

Biological Background: Importin Subunit Alpha-7 Function and Localization

• Importin subunit alpha-7 (KPNA6) is a member of the karyopherin alpha family that functions as an adapter for nuclear protein import by linking cargo to the importin beta-1 (KPNB1) transport receptor.
• It specifically recognizes substrates bearing classical nuclear localization signals (NLSs), including both monopartite and bipartite motifs, via its armadillo (ARM) repeat domain.
• The resulting importin-alpha/beta complex docks at the nuclear pore complex (NPC) through interactions between KPNB1 and nucleoporin FG repeats, enabling subsequent translocation.
• Nuclear import is energy-dependent and requires the small GTPase Ran; directionality is maintained by a RanGTP gradient across the nuclear envelope, with high RanGTP in the nucleus promoting complex disassembly.
• KPNA6 is widely expressed across human tissues, consistent with its fundamental role in constitutive nucleocytoplasmic transport.
• Post‑translational modifications include acetylation and phosphorylation, and the protein participates in host‑virus interactions, highlighting its regulatory and pathophysiological significance.

Experimental Guidance and Technical Tips

• The immunogen was designed from a conserved region (aa 69‑169) of human KPNA6, which likely contributes to the observed cross‑reactivity with mouse and rat orthologs.
• For western blotting, a band near 60 kDa is expected; consider titrating the antibody and including a whole‑cell lysate control to verify specificity.
• In immunohistochemistry (IHC‑P), antigen retrieval conditions and antibody concentration should be optimized for the tissue type and fixation protocol.
• The antibody may also be suitable for ELISA‑based assays; validate binding in the relevant sample matrix to ensure compatibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research laboratories with a comprehensive portfolio of IVD raw materials, including optimized antibodies for nucleocytoplasmic transport targets like KPNA6. We offer reliable supply of monoclonal and polyclonal antibodies, validated antibody pairs, and bulk ancillary reagents to streamline your assay development. Our sourcing expertise also covers rare target raw materials, ensuring continuity from concept to clinic.