Anti-KPNA6 Monoclonal Antibody for WB - O60684

Core Product Specifications and Parameters

Biological Background: Importin Subunit Alpha-7 (KPNA6) Function and Localization

• KPNA6, also known as importin subunit alpha-7 or karyopherin subunit alpha-6, is a member of the importin alpha family, which mediates the nuclear import of proteins.
• It functions as an adapter that recognizes and binds to nuclear localization signals (NLS) on cargo proteins, facilitating their transport into the nucleus via interaction with importin-beta (KPNB1).
• The importin alpha/beta-cargo complex docks at the nuclear pore complex (NPC) through binding of KPNB1 to nucleoporin FxFG repeats, and is translocated through the pore in a Ran-GTP-dependent manner.
• At the nucleoplasmic side, Ran-GTP binding to importin-beta triggers complex disassembly, with importin alpha and beta recycled back to the cytoplasm after GTP hydrolysis.
• KPNA6 is widely expressed in tissues, consistent with its fundamental role in nucleocytoplasmic trafficking.
• Post-translational modifications of KPNA6 include acetylation and phosphorylation, which may regulate its activity or interactions.
• The protein plays a role in host-virus interactions, as viral proteins often hijack host importins to enter the nucleus.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 69-169 of human KPNA6 (NP_036448.1); this region is likely conserved and may contribute to the antibody's cross-reactivity with mouse and rat.
• The antibody is validated for Western blotting (WB), immunohistochemistry (IHC-P), immunofluorescence (IF/ICC), and ELISA; consider appropriate sample preparation and detection protocols for each application.
• For WB, the predicted molecular weight of KPNA6 is 60 kDa; use appropriate molecular weight markers and verify band specificity in relevant lysates.
• Since KPNA6 is widely expressed, note potential endogenous expression in many cell lines when designing controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For KPNA6-related research and immunoassay development, we offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to streamline your procurement and accelerate your diagnostics pipeline.