Anti-KPNA6 Monoclonal Antibody for WB, IF/ICC, ELISA - O60684

Core Product Specifications and Parameters

Biological Background: KPNA6 Function and Localization

• Importin subunit alpha-7 (KPNA6) is a member of the importin alpha family and functions as an adapter for the nuclear transport receptor karyopherin subunit beta-1 (KPNB1).
• It specifically recognizes and binds to cargo proteins containing either a simple or bipartite nuclear localization signal (NLS).
• The importin alpha/beta heterodimer docks at the nuclear pore complex (NPC) via KPNB1 binding to FG-repeat nucleoporins, and translocation through the NPC is driven by a RanGTP-dependent mechanism.
• Directional nuclear import is conferred by the asymmetric distribution of RanGTP (high in the nucleus) and RanGDP (high in the cytoplasm), leading to cargo release in the nucleus.
• After cargo release, importin alpha and beta are recycled back to the cytoplasm for another round of transport.
• KPNA6 is widely expressed across tissues.
• Post-translational modifications include acetylation and phosphorylation; the protein is also implicated in host-virus interactions.
• The predicted molecular weight is approximately 60 kDa (60030 Da).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 69–169 of human KPNA6; consider performing a peptide competition or knockdown experiment to confirm band specificity in your system.
• For Western blot, a starting dilution of 1:1000–1:2000 may be used, but optimal concentration should be determined empirically.
• For immunofluorescence (IF/ICC), include appropriate fixation/permeabilization steps; test both methanol and paraformaldehyde protocols.
• Cross-reactivity with human and mouse is specified; when using mouse samples, verify antibody specificity as endogenous IgG may interfere if using anti-mouse secondary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our KPNA6 Rabbit mAb targets importin alpha-7, a key player in nuclear transport and cell biology research. Whether you need rare-target raw materials, custom antibody production, or technical consulting—from concept to clinic—CamelBio provides end-to-end support for diagnostic manufacturers and research laboratories.