Anti-KPNA5 Polyclonal Antibody for WB, ELISA - O15131

Core Product Specifications and Parameters

Biological Background: KPNA5 Function and Localization

• Importin subunit alpha-6 (KPNA5), also known as karyopherin subunit alpha-5, is a member of the importin alpha family that mediates nucleocytoplasmic transport.
• It functions as an adapter that binds classical nuclear localization signals (NLS) on cargo proteins and links them to importin beta (KPNB1) for translocation through the nuclear pore complex in a RanGTP-dependent manner.
• KPNA5 also recognizes non-classical NLSs on proteins like STAT1 and STAT2, and viral components such as influenza A nucleoprotein and human cytomegalovirus UL84, contributing to both cellular signaling and viral replication.
• The protein is predominantly cytoplasmic and shuttles between the nucleus and cytoplasm.
• It is specifically expressed in testis, suggesting a role in spermatogenesis or testis-specific transport processes.
• KPNA5 contains armadillo (ARM) repeats that form the NLS-binding groove; repeats 7–9 are particularly involved in viral protein recognition.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1–240) of human KPNA5; verify that the antibody recognizes endogenous protein in your target species, as epitope accessibility may vary.
• For Western blot (WB), use lysates from testis-derived cell lines or tissues known to express KPNA5; a band near 61 kDa is expected based on predicted molecular weight.
• In ELISA, consider using recombinant KPNA5 protein as a standard and optimize coating conditions; the antibody may function as a capture or detection reagent in sandwich pairs.
• Cross-reactivity with mouse and rat has been indicated, but validate in your specific sample matrix, as cross-reactivity can depend on sequence homology and sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti-KPNA5, and bulk ancillary reagents. Whether you're developing immunoassays for nuclear transport research or viral interaction studies, our supply chain ensures timely access to high-quality materials. Partner with us to streamline your diagnostic development from concept to clinic.