Anti-KPNA3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O00505

Core Product Specifications and Parameters

Biological Background: Importin subunit alpha-4 Function and Localization

• Importin subunit alpha-4 (also known as karyopherin subunit alpha-3, importin alpha Q2/Qip2, SRP1-gamma) is a member of the importin alpha family that functions as an adapter for nuclear transport.
• It binds specifically to nuclear localization signals (NLS), either simple or bipartite, on cargo proteins and links them to importin-beta (KPNB1) for translocation through the nuclear pore complex.
• The nuclear import cycle is regulated by Ran GTPase, with directionality conferred by the asymmetric distribution of GTP- and GDP-bound Ran across the nuclear envelope.
• It is localized in both the cytoplasm and the nucleus, shuttling between these compartments as part of the importin recycling process.
• In vitro, it mediates the nuclear import of human cytomegalovirus UL84 via a non-classical NLS and recognizes the NLS of influenza A virus nucleoprotein, highlighting its role in host-virus interactions.
• It is ubiquitously expressed, with highest levels in heart and skeletal muscle, and is subject to acetylation and phosphorylation.
• Mutations in KPNA3 have been linked to hereditary spastic paraplegia (SPG88), suggesting a role in neuronal maintenance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-210) of human KPNA3; consider its potential to recognize all reported splice isoforms.
• For Western blotting (WB), the predicted molecular weight is approximately 58 kDa; a clear band may be observed in lysates from tissues with high expression, such as heart or skeletal muscle.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), both cytoplasmic and nuclear localization patterns are expected; optimize antigen retrieval and permeabilization conditions accordingly.
• Cross-reactivity with mouse and rat KPNA3 allows for studies in common rodent models; however, confirm detection efficiency in the relevant species and tissue type.
• When using this antibody for ELISA, ensure proper coating and detection conditions, and consider pairing with appropriate secondary reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs and bulk ancillary reagents, to support your diagnostic assay development. Our KPNA3 rabbit polyclonal antibody enables research into nuclear transport mechanisms and viral host interactions, with one-stop sourcing that bridges the gap from concept to clinical application. We specialize in providing optimized polyclonal and monoclonal antibodies for rare targets, along with technical consulting to accelerate your project.