Anti-KPNA3 Rabbit Polyclonal Antibody for WB and ELISA - O00505

Core Product Specifications and Parameters

Biological Background: KPNA3 Function and Localization

• KPNA3 encodes importin subunit alpha-4, also known as karyopherin alpha-3, SRP1-gamma, and Qip2, acting as an adapter for nuclear protein import.
• In the classical import pathway, importin alpha (KPNA3) recognizes nuclear localization signals (NLS) on cargo proteins and links them to importin beta (KPNB1), which then docks at the nuclear pore complex.
• The import complex translocates through the nuclear pore via interactions with nucleoporin FxFG repeats, with directionality driven by the Ran GTPase gradient.
• Besides cargo transport, KPNA3 facilitates the nuclear entry of viral proteins, including human cytomegalovirus UL84 and influenza A nucleoprotein, highlighting its role in host-virus interactions.
• KPNA3 is ubiquitously expressed, with highest levels in heart and skeletal muscle, and localizes to both cytoplasm and nucleus, consistent with its shuttling function.
• Post-translational modifications include acetylation and phosphorylation, and the protein is implicated in hereditary spastic paraplegia (SPG88) and neurodegeneration.
• The gene is associated with keywords including protein transport, viral penetration into host nucleus, and disease variant, reflecting its diverse biological roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-210) of human KPNA3, which contains part of the importin-beta binding (IBB) domain; this region may influence antibody specificity for importin complex detection.
• For Western blot, a band around 58 kDa is expected for human KPNA3; consider using appropriate sample preparation and reducing conditions to visualize the protein.
• In ELISA applications, the antibody may be used for capture or detection; titrate to determine optimal working concentration in your assay system.
• Validate cross-reactivity with other importin alpha family members if required, as high homology could lead to cross-reactivity.
• For studying nuclear-cytoplasmic shuttling, consider subcellular fractionation followed by WB to confirm localization changes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents for IVD development. This anti-KPNA3 antibody is part of our portfolio for studying nucleocytoplasmic transport and neurodegenerative disease markers. Contact our team for custom sourcing of rare targets, recombinant proteins, and technical consultation to accelerate your assay development.