Anti-KPNA2 Rabbit Monoclonal Antibody for WB and ELISA - P52292

Core Product Specifications and Parameters

Biological Background: Importin subunit alpha-1 Function and Localization

• KPNA2 acts as an adapter protein linking NLS-containing cargo to KPNB1 (importin-beta) for nuclear import, recognizing both simple and bipartite nuclear localization signals. Related references: PMID:28991411, PMID:32130408, PMID:7604027
• The docking of the importin-cargo complex to the nuclear pore complex is mediated by KPNB1 binding to FxFG nucleoporin repeats, with subsequent translocation driven by the Ran GTPase cycle. Related references: PMID:7604027, PMID:7754385
• KPNA2 contributes to the nuclear import of the PR-DUB complex component BAP1, functioning redundantly with KPNA1 and Transportin-1. Related references: PMID:35446349
• Subcellularly, KPNA2 localizes to the cytoplasm and nucleus, and is also associated with membranes of the endoplasmic reticulum and Golgi apparatus.
• KPNA2 is expressed ubiquitously, consistent with its fundamental role in nuclear transport.
• The protein is subject to acetylation and phosphorylation, and is involved in host-virus interactions, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal 1–100 amino acids of human KPNA2, a region conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, expect a band near 58 kDa; using nuclear and cytoplasmic fractionation may help confirm localization.
• In ELISA applications, consider coating with recombinant KPNA2 protein or the antigenic peptide for antibody capture assays.
• Validate antibody performance in the relevant sample system and experimental context, as reactivity may vary with conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For studies on nucleocytoplasmic transport and importin biology, this KPNA2 rabbit monoclonal antibody is an ideal tool for western blotting and ELISA applications. CamelBio can supply optimized antibody pairs, recombinant KPNA2 proteins, and other ancillary reagents to accelerate your research and assay development.