Anti-KPNA2 Polyclonal Antibody for WB/IP/ELISA - P52292

Core Product Specifications and Parameters

Biological Background: KPNA2 Function and Localization

• KPNA2 (Importin subunit alpha-1) is a member of the karyopherin alpha family, functioning as an adapter that recognizes nuclear localization signals (NLS) on cargo proteins and mediates their import through the nuclear pore complex in complex with importin-beta (KPNB1). Related references: PMID:7604027, PMID:7754385, PMID:28991411, PMID:32130408
• In addition to its cytoplasmic and nuclear localization, KPNA2 has been detected at the endoplasmic reticulum and Golgi apparatus membranes, suggesting roles in organelle-associated transport processes.
• KPNA2 acts redundantly with KPNA1 and TNPO1 in mediating the nuclear import of specific cargos, such as the deubiquitinase BAP1 from the PR-DUB complex. Related references: PMID:35446349
• The protein undergoes multiple post-translational modifications including acetylation, phosphorylation, and ubiquitination, which may regulate its activity and interactions.
• KPNA2 is ubiquitously expressed in human tissues, consistent with its essential role in constitutive nuclear transport.
• KPNA2 is implicated in host-virus interactions, where viruses may exploit the importin pathway for nuclear entry.
• The protein structure contains Armadillo (ARM) repeats, and its three-dimensional structure has been resolved, providing insights into NLS recognition.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 290 amino acids of human KPNA2, which includes the importin-beta binding domain; this region is highly conserved among vertebrates, supporting cross-reactivity with mouse and potentially other species.
• For Western blot (WB), the predicted molecular weight of KPNA2 is ~58 kDa; consider using appropriate lysis buffers and loading controls for nuclear/cytoplasmic extracts to optimize detection.
• In immunoprecipitation (IP) experiments, verify antibody compatibility with native protein conformations and include appropriate negative controls to confirm specificity.
• ELISA assays may require optimization of coating antigen and antibody dilution; consider using recombinant KPNA2 protein as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-KPNA2 antibody, to support nuclear transport research and diagnostic development. Our one-stop sourcing model also provides bulk ancillary reagents and access to rare-target raw materials, streamlining your path from concept to clinic. Reach out to explore how CamelBio can accelerate your next project.