Anti-[KO Validated] PD-1/CD279 Rabbit Polyclonal Antibody for WB, ELISA - Q15116

Core Product Specifications and Parameters

Biological Background: PD-1/CD279 Function and Localization

• PD-1/CD279 is an inhibitory receptor on antigen-activated T-cells that is critical for maintaining immune tolerance to self. Related references: PMID:21276005, PMID:31754127, PMID:32184441
• It delivers inhibitory signals upon binding to its ligands CD274 (PD-L1) and CD273 (PD-L2). Related references: PMID:21276005, PMID:26602187
• Following TCR engagement, PD-1 associates with the TCR-CD3 complex at the immunological synapse and directly suppresses T-cell activation. Related references: PMID:32184441
• The inhibitory mechanism involves phosphorylation of the ITSM motif and recruitment of PTPN11/SHP-2, which dephosphorylates key signaling molecules like ZAP70 and CD3zeta. Related references: PMID:32184441
• Tumors exploit this pathway to evade immune destruction; PD-L1 binding inhibits CTL effector function, promoting tumor survival. Related references: PMID:28951311
• Blocking PD-1/PD-L1 interaction reverses T-cell exhaustion and restores anti-tumor immunity, underpinning checkpoint inhibitor therapies. Related references: PMID:22658127, PMID:25034862, PMID:25399552
• PD-1 localizes to the cell membrane and is a transmembrane glycoprotein with immunoglobulin-like domains, disulfide bonds, and regulatory phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 21-170 of human PD-1, spanning the extracellular domain, which may facilitate recognition of both soluble and membrane-bound forms.
• For Western blot, a band around 32-35 kDa is expected under reducing conditions; consider using KO-validated samples to confirm specificity.
• For ELISA, titrate antibody concentration and validate using antigen-coated plates or cell-based assays with PD-1-expressing cells.
• Given cross-reactivity to mouse and rat, this antibody can be a useful tool for cross-species studies, but performance should be verified in each species' sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. This anti-PD-1/CD279 antibody complements our portfolio of immune checkpoint reagents, and we can supply validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents in bulk to streamline your assay development. For custom or rare-target sourcing needs, our team is ready to assist.