Anti-[KO Validated] Edf1 Rabbit Polyclonal Antibody for WB and ELISA - Q9JMG1

Core Product Specifications and Parameters

Biological Background: Edf1 Function and Localization

• Edf1, also known as multiprotein-bridging factor 1 (MBF1), is a 148-amino acid protein encoded by the Edf1 gene in mouse (GeneID: 59022).
• It functions as a transcriptional coactivator, enhancing the activity of NR5A1, ligand-dependent NR1H3/LXRA, and PPARG.
• Edf1 potentiates the DNA-binding activity of transcription factors ATF1, ATF2, CREB1, and NR5A1.
• The protein regulates nitric oxide synthase activity, likely by sequestering calmodulin in the cytoplasm.
• Edf1 may play roles in endothelial cell differentiation, hormone-induced cardiomyocyte hypertrophy, and lipid metabolism.
• It localizes to both the cytoplasm and nucleus, with detected expression in brain, liver, kidney, heart, and testis.
• Post-translational modifications include acetylation, phosphorylation, and methylation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• This antibody was raised against full-length mouse Edf1 (residues 1–148). In Western blot, a band at approximately 16 kDa is expected.
• For knockout validation, ensure appropriate knockout cell or tissue lysates are used to confirm specificity.
• When performing ELISA, optimize coating conditions and include proper negative controls to account for polyclonal background.
• Given the reported expression in multiple tissues, consider using relevant positive control lysates (e.g., mouse brain, liver, or heart) for initial testing.

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