Anti-[KO Validated] Calpain 2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P17655

Core Product Specifications and Parameters

Biological Background: Calpain-2 Function and Localization

• Calpain-2, also known as M-calpain, is the large catalytic subunit of the calcium-activated neutral protease calpain-2, encoded by the CAPN2 gene. It belongs to the peptidase C2 family and functions as a heterodimer with a small regulatory subunit.
• As a calcium-regulated non-lysosomal thiol-protease, it catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction, playing a role in cell migration, proliferation, and differentiation.
• Calpain-2 proteolytically cleaves myocilin (MYOC) at Arg-226, a cleavage event implicated in the regulation of intraocular pressure (PubMed:17650508). Related references: PMID:17650508
• Following neuronal stimulation, calpain-2 cleaves CPEB3, which abolishes its translational repressor activity, thereby enabling translation of CPEB3 target mRNAs (By similarity).
• Subcellular localization is primarily cytoplasmic and associated with the cell membrane, consistent with its calcium-dependent activation and translocation.
• Tissue expression is ubiquitous, reflecting its fundamental role in cellular proteolytic processes across multiple organs and cell types.
• Post-translational modifications include acetylation, phosphorylation, and autocatalytic cleavage, which regulate its activity and function. It contains key structural domains such as calpain catalytic and calcium-binding domains, and is classified under thiol protease and hydrolase families.

Experimental Guidance and Technical Tips

• This antibody was generated using a recombinant fusion protein spanning amino acids 350–680 of human Calpain-2 (NP_001739.2), a region covering part of the catalytic domain and the C2-like calcium-binding domain. Consider this immunogen design when validating reactivity in your model system.
• For Western blot (WB) applications, expect a band at the predicted molecular weight of ~80 kDa. Note that calpain-2 may undergo autocatalytic processing, which could result in additional lower molecular weight fragments.
• The antibody is also suitable for immunohistochemistry (IHC-P) and ELISA. When performing IHC, include appropriate controls and optimize antigen retrieval conditions for the tissues of interest.
• Cross-reactivity has been observed with human, mouse, and rat samples. For other species, verify sequence homology, particularly in the immunogen region, before use.
• Since calpain-2 activity is calcium-dependent, consider the calcium concentration in your lysis buffers and experimental conditions, as it may affect the protease's autolysis and epitope availability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a streamlined sourcing solution for IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our Calpain-2 rabbit polyclonal antibody supports studies in cytoskeletal remodeling, signal transduction, and protease-related pathologies. With comprehensive technical services from concept to clinic, we help accelerate your assay development and procurement of rare-target raw materials.