Anti-KNTC1 Rabbit Polyclonal Antibody for WB, ELISA - P50748

Core Product Specifications and Parameters

Biological Background: KNTC1 Function and Localization

• KNTC1 (Kinetochore-associated protein 1), also known as Rough deal homolog (ROD), is encoded by the KNTC1 gene and is conserved across eukaryotes.
• Essential component of the mitotic checkpoint; required for assembly of dynein-dynactin and MAD1-MAD2 complexes onto kinetochores, and functions within the mitotic RZZ complex. Related references: PMID:11146660 PMID:11590237 PMID:15824131
• Subcellular localization: Predominantly cytoplasmic but also nuclear, concentrates at centromeres/kinetochores and spindle microtubules during mitosis.
• Tissue specificity: Highly expressed in testis, with lower expression in other tissues.
• Post-translational modifications: Phosphoprotein; phosphorylation may regulate its function during cell cycle.
• Keywords: Alternative splicing produces multiple isoforms; involved in cell division, mitosis, and centromere/kinetochore structure.
• Theoretical molecular weight: ~251 kDa.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a recombinant fragment corresponding to amino acids 900–1100 of human KNTC1, a central region potentially exposed in native conformation.
• For WB, anticipate a band around 251 kDa; use low-percentage SDS-PAGE gels and extended transfer times for high-molecular-weight proteins.
• Validate antibody performance in KNTC1-expressing cell lines (e.g., HeLa, HEK293) or tissues such as testis where expression is high.
• For ELISA, test serial dilutions to establish optimal working concentration; use recombinant KNTC1 or peptide as coating antigen.
• Consider testing cross-reactivity in rat samples as indicated by the supplier.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, bulk reagents, and rare-target sourcing. This anti-KNTC1 antibody supports cell cycle and mitotic checkpoint research applications. Through one-stop procurement and technical support, CamelBio accelerates the transition from concept to clinical development.