Anti-KMT5A/SETD8 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NQR1

Core Product Specifications and Parameters

Biological Background: KMT5A/SETD8 Function and Localization

• Functions as a protein-lysine N-methyltransferase that monomethylates both histone and non-histone proteins. Related references: PMID:12086618, PMID:12121615, PMID:15964846
• Specifically monomethylates histone H4 at Lys-20 (H4K20me1), a modification enriched during mitosis and linked to epigenetic transcriptional repression. Related references: PMID:12086618, PMID:12121615, PMID:15200950
• Acts predominantly in euchromatin regions to silence euchromatic genes; essential for cell proliferation and maintaining higher-order DNA structure during mitosis. Related references: PMID:12086618, PMID:12121615, PMID:15200950
• Involved in chromosome condensation and proper cytokinesis. Related references: PMID:12086618, PMID:12121615, PMID:15200950
• Prefers nucleosomes as substrate over free histones. Related references: PMID:12086618, PMID:12121615, PMID:15200950
• Monomethylates p53/TP53 at Lys-382, repressing p53 target genes. Related references: PMID:17707234
• Regulates TGF-beta signaling negatively and promotes cell migration. Related references: PMID:23478445
• Localizes to the nucleus and chromosomes.
• Undergoes post-translational modifications, including acetylation, phosphorylation, and ubiquitination, which may regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 73–352, encompassing part of the SET catalytic domain; consider this region’s conservation when assessing cross-reactivity.
• For Western blot, the expected molecular weight is approximately 43 kDa; validate band specificity in a relevant cellular extract.
• In immunofluorescence/ICC, anticipate nuclear and chromosomal localization; optimize fixation and permeabilization to preserve epitope accessibility.
• ELISA can be used for detection or quantification; titrate the antibody and confirm performance in a defined antigen system.
• When probing samples from mouse or rat, confirm cross-reactivity by comparing staining patterns with known human controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and laboratories streamlined access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For epigenetic targets like KMT5A/SETD8, our one-stop sourcing capabilities cover the full development chain—from candidate evaluation to supply chain delivery—helping you accelerate assay design and clinical translation.