Anti-KMT5A/SETD8 Rabbit Monoclonal Antibody (KD Validated) for WB, ELISA - Q9NQR1

Core Product Specifications and Parameters

Biological Background: KMT5A/SETD8 Function and Localization

• KMT5A/SETD8 is an N-lysine methyltransferase that monomethylates both histone and non-histone proteins, playing a critical role in epigenetic regulation.
• It specifically monomethylates histone H4 at Lys-20 (H4K20me1), a mark enriched during mitosis and associated with transcriptional repression in euchromatin.
  Related references:
  PMID:12086618
  PMID:12121615
  PMID:15200950
• The enzyme prefers nucleosomal substrates over free histones, underscoring its role in chromatin biology.
  Related references:
  PMID:12086618
  PMID:12121615
  PMID:15200950
• KMT5A is required for proper cell proliferation, chromosome condensation, and cytokinesis, likely by maintaining higher-order DNA structure during mitosis.
  Related references:
  PMID:12086618
  PMID:12121615
  PMID:15200950
• Beyond histones, it monomethylates p53/TP53 at Lys-382, contributing to the repression of p53 target genes.
  Related references:
  PMID:17707234
• It also negatively regulates TGF-beta signaling and promotes cell migration.
  Related references:
  PMID:23478445
• KMT5A is subject to acetylation, phosphorylation, and ubiquitination, as indicated by proteomics and structural studies. These modifications may regulate its activity and stability.
• Subcellularly, KMT5A localizes to the nucleus and associates with chromosomes, consistent with its functions in chromatin modification and mitotic regulation.

Experimental Guidance and Technical Tips

• When performing Western blot, a band around 43 kDa is expected for full-length KMT5A/SETD8. Ensure adequate nuclear extraction protocols to enrich the target protein.
• Since the antibody is KD-validated, consider using knockdown or knockout samples as negative controls to confirm specificity in your experimental system.
• For ELISA, the immunizing peptide (aa 1–100) can be used as a positive control; standard sandwich or indirect ELISA protocols may be applied.
• Although recommended for human and mouse, cross-reactivity with other species is not confirmed; validate if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide validated IVD raw materials, including this anti-KMT5A/SETD8 rabbit monoclonal antibody, ideal for epigenetics and cell cycle studies. Beyond single antibodies, we offer optimized paired reagents, bulk ancillary supplies, and custom sourcing for rare targets—supporting your workflow from concept to clinic. Reach out to explore how our one-stop procurement solutions can accelerate your assay development.