Anti-KMT5A/SETD8 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NQR1

Core Product Specifications and Parameters

Biological Background: KMT5A/SETD8 Function and Localization

• KMT5A (also known as SETD8, PR-Set7) is a protein-lysine N-methyltransferase that monomethylates both histone and non-histone proteins, including histone H4 at Lys-20 (H4K20me1) and p53 at Lys-382. Related references: PMID:12086618 PMID:12121615 PMID:15964846
• H4K20me1 is a specific epigenetic mark enriched during mitosis and associated with transcriptional repression. KMT5A primarily functions in euchromatin regions to silence euchromatic genes. Related references: PMID:12086618 PMID:12121615 PMID:15200950
• KMT5A is required for cell proliferation, playing a role in maintaining higher-order DNA structure during mitosis, chromosome condensation, and proper cytokinesis. Nucleosomes are preferred substrates over free histones. Related references: PMID:12086618 PMID:12121615 PMID:15200950
• The enzyme mediates monomethylation of p53/TP53 at Lys-382, leading to repression of p53 target genes, and plays a negative role in TGF-beta response while positively regulating cell migration. Related references: PMID:17707234 PMID:23478445
• KMT5A belongs to the SET domain-containing methyltransferase family and contains a PR/SET domain, essential for its catalytic activity.
• The protein localizes to the nucleus and specifically associates with chromosomes, consistent with its role in chromatin regulation.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation, which may regulate its stability, activity, or localization. Alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen used to generate this rabbit polyclonal antibody is a recombinant fusion protein spanning amino acids 1-393 of human KMT5A/SETD8, representing the full-length protein. This design may yield antibodies recognizing multiple epitopes and potentially detecting various isoforms or post-translational modifications.
• For Western blot (WB), the predicted molecular weight of human KMT5A is approximately 43 kDa. Ensure appropriate blocking and antibody dilution optimization for your specific sample type.
• In immunofluorescence/immunocytochemistry (IF/ICC), KMT5A is expected to localize predominantly in the nucleus with chromatin association. Validate nuclear staining by co-staining with a nuclear marker.
• For ELISA applications, consider using recombinant KMT5A protein as a coating antigen and cross-validate specificity with relevant controls. Note that this antibody cross-reacts with mouse and rat KMT5A, which share high sequence homology with human.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material sourcing for diagnostic manufacturers, labs, and research institutes, providing a single point of access to antibodies, antigens, and ancillary reagents. The KMT5A/SETD8 antibody supports epigenetic and cell cycle research applications, and CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk reagents for large-scale assay development. For rare or custom targets, our expert team assists with raw-material sourcing to accelerate your transition from concept to clinic.