Anti-KMT1B / SUV39H2 Rabbit Monoclonal Antibody for WB, ELISA - Q9H5I1

Core Product Specifications and Parameters

Biological Background: KMT1B/SUV39H2 Function and Localization

• SUV39H2 (also known as KMT1B) is a histone-lysine N-methyltransferase that specifically catalyzes trimethylation of 'Lys-9' of histone H3 (H3K9me3), a hallmark of repressive heterochromatin. Related references: PMID:10949293
• The enzyme uses monomethylated H3 'Lys-9' as substrate and provides a binding platform for HP1 proteins (CBX1, CBX3, CBX5), linking histone methylation to chromatin compaction and gene silencing.
• SUV39H2 is a central player in establishing constitutive heterochromatin at pericentric and telomeric regions, and its activity is required for directing DNA methylation at pericentric repeats. Related references: PMID:10949293 PMID:41094145
• It interacts with the retinoblastoma protein RB1, which targets it to chromatin and contributes to cell cycle regulation, transcriptional repression, and telomere length control. Related references: PMID:14765126
• SUV39H2 localizes predominantly to the nucleus and concentrates at centromeric heterochromatin (as annotated in UniProt).
• By similarity, it may participate in higher-order chromatin reorganization during spermatogenesis and is recruited by the large PER complex to circadian gene promoters to impose a repressive chromatin state.
• The protein belongs to the SET domain methyltransferase family and contains characteristic pre-SET, SET, and post-SET domains, underscoring its enzymatic function.

Experimental Guidance and Technical Tips

• The immunogen spans residues 311–410 of human SUV39H2, a region predicted to be accessible; verify specificity and performance in your target samples, as isoforms or post-translational modifications may affect binding.
• For Western blotting, a band near 47 kDa is expected; consider using nuclear extracts or whole cell lysates from cell lines known to express SUV39H2.
• Optimize antibody dilution for ELISA by performing checkerboard titrations against your specific antigen or coated peptide.
• As with any antibody, validate detection in your experimental context using appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This high-quality anti-KMT1B/SUV39H2 monoclonal antibody is a powerful tool for epigenetic and gene regulation research, exemplifying our capability to supply validated antibodies for critical targets. For your specific IVD development needs, explore our portfolio of validated antibody pairs, bulk monoclonals, and ancillary reagents, and leverage our expertise in rare target sourcing.