Anti-KLRD1 Polyclonal Antibody for WB and ELISA - Q13241

Core Product Specifications and Parameters

Biological Background: KLRD1 Function and Localization

• KLRD1 (CD94) is a type II integral membrane glycoprotein belonging to the killer cell lectin-like receptor family. It functions as a ligand-binding subunit that pairs with KLRC1 or KLRC2 to form functional heterodimers.
• The KLRD1-containing complex recognizes the non-classical MHC class Ib molecule HLA-E loaded with self-peptides, enabling immune cells to monitor MHC class I expression and maintain self-tolerance. Related references: PMID:10023772, PMID:18064301, PMID:18083576
• The KLRD1-KLRC1 complex transmits inhibitory signals through ITIM-mediated recruitment of SHIP-1 and SHIP-2 phosphatases, counteracting activating receptor signals. This inhibitory function regulates NK and T cell activation, and in tumor microenvironments may contribute to immune cell exhaustion. Related references: PMID:30860984, PMID:9430220, PMID:9485206, PMID:12387742, PMID:18064301, PMID:30503213, PMID:12165520
• The KLRD1-KLRC2 complex acts as an activating receptor, signaling through the DAP12 adapter protein upon HLA-E binding. This pathway is important for adaptive NK cell responses and antiviral immunity. Related references: PMID:15940674, PMID:9655483, PMID:30134159, PMID:9754572, PMID:20952657, PMID:21825173
• KLRD1 is primarily located on the cell membrane and is expressed in NK cells, subsets of CD8-positive T cells, and intraepithelial lymphocytes. Its expression is differentially regulated in various lymphocyte populations. Related references: PMID:21825173, PMID:9430220, PMID:9485206, PMID:12387742, PMID:20952657
• The protein undergoes post-translational modifications, including disulfide bond formation and glycosylation.
• Viruses such as human cytomegalovirus and HIV-1 exploit HLA-E/KLRD1 interactions to evade NK cell cytotoxicity. SARS-CoV-2 may also induce NK cell exhaustion through this pathway. Related references: PMID:32859121

Experimental Guidance and Technical Tips

• The immunogen (amino acids 32-179) encompasses the extracellular C-type lectin domain, which is predicted to be accessible for antibody binding.
• For Western blot analysis, the predicted molecular weight is 21 kDa; however, glycosylation may result in a higher apparent molecular weight.
• Validate the antibody in positive control lysates from NK cell lines (e.g., NK-92) or primary human NK cells. Cross-reactivity with mouse allows testing in mouse splenocyte or NK cell lysates.
• For ELISA applications, the antibody can be used for antigen capture or detection following standard indirect ELISA protocols. Optimization of coating and detection conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, including this anti-KLRD1 polyclonal antibody suited for immunology and infectious disease research. As a one-stop sourcing partner, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assistance with rare target procurement. Whether you need consistent supply for diagnostic kit development or specialized raw materials for NK cell and immune checkpoint studies, CamelBio bridges your concept to clinic.