Anti-KLRC4 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - O43908

Core Product Specifications and Parameters

Biological Background: KLRC4 Function and Localization

• KLRC4 encodes NKG2-F, a type II integral membrane protein also known as NKG2F or NKG2-F-activating NK receptor.
• It belongs to the NKG2 family of C-type lectin-like receptors expressed primarily on natural killer (NK) cells.
• NKG2-F may function as a receptor for MHC class I HLA-E molecules, contributing to NK cell recognition and immune surveillance.
• The protein contains a signal-anchor transmembrane domain and is localized to the cell membrane.
• Post-translational modifications include at least one disulfide bond, consistent with its structure as a C-type lectin-like receptor.
• No tissue expression outside NK cells has been reported, indicating a specialized role in innate immunity.

Experimental Guidance and Technical Tips

• For Western blot, consider using this polyclonal antibody to detect the ~18 kDa KLRC4 protein in NK cell lysates; optimize loading and detection conditions.
• When performing immunofluorescence (IF/ICC or IF-P), validate fixation and permeabilization protocols, as the target is a transmembrane protein.
• The antibody cross-reacts with mouse and rat KLRC4; verify specificity in these species using appropriate positive and negative controls.
• For ELISA applications, titrate the antibody and use a suitable coating antigen to establish a sensitive detection system.
• Given that the immunogen spans the N-terminal region (aa 1–55), the antibody likely recognizes epitopes within the cytoplasmic domain; consider this for epitope mapping experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing experience for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom procurement for rare targets. With extensive supply chain capabilities in immunology and NK cell receptor research, we help diagnostic manufacturers and laboratories efficiently access the critical reagents needed for assay development and biomarker studies. Contact us to discuss how we can support your next project, from concept to clinical application.