Anti-KLKB1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P03952

Core Product Specifications and Parameters

Biological Background: KLKB1 Function and Localization

• KLKB1 encodes plasma prekallikrein, the zymogen of plasma kallikrein, a serine protease that circulates in blood as an inactive precursor.
• Upon activation, plasma kallikrein participates in the surface-dependent activation of blood coagulation by reciprocally activating coagulation factor XII (FXII) after binding to negatively charged surfaces.
• It releases the vasoactive peptide bradykinin from high-molecular-weight kininogen (HMWK), linking the contact activation system to inflammation and vascular permeability.
• Plasma kallikrein may also contribute to the renin-angiotensin system by converting prorenin into active renin.
• Subcellular localization is exclusively extracellular; the protein is secreted into plasma, where it is normally found.
• The protein contains a signal peptide, multiple Apple domains for protein–protein interactions, and a trypsin-like serine protease domain; it is N-glycosylated and stabilized by disulfide bonds.
• Genetic variants in KLKB1 are associated with prekallikrein deficiency (Fletcher trait), a rare condition with prolonged activated partial thromboplastin time but typically no bleeding tendency.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 20–300 of the heavy chain, which contains the four Apple domains responsible for binding to HMWK and negatively charged surfaces; this region is highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, a band near 71 kDa (prekallikrein) or ~85 kDa (full-length with glycosylation) may be detected; reduction with DTT/TCEP is recommended to resolve disulfide-linked chains.
• In immunohistochemistry (IHC-P), consider antigen retrieval with citrate buffer (pH 6.0) and a detection system sensitive for rabbit primary antibodies; pre-immune serum or blocking peptide controls are advised to confirm specificity.
• For ELISA, the antibody can be used as capture or detection reagent; however, pairing with a monoclonal antibody to a distinct epitope may improve sensitivity and specificity.
• Because plasma kallikrein is abundant in blood, pre-adsorption of the polyclonal antibody against immobilized mouse/rat serum proteins can reduce background in rodent tissue applications if non-specific binding is observed.
• Validate performance in your specific sample type (plasma, serum, tissue lysate) by including positive controls (e.g., purified plasma kallikrein or activated plasma) and negative controls (KLKB1-depleted plasma or knockout cell lines if available).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For coagulation and contact system research, we can also source rare targets and custom immunogens upon request, supporting projects from concept to clinic with reliable supply and technical consulting.