Anti-KLK3 Rabbit Polyclonal Antibody for WB - P07288

Core Product Specifications and Parameters

Biological Background: KLK3 Function and Localization

• KLK3, also known as Prostate-Specific Antigen (PSA) or Kallikrein-3, is a secreted serine protease that belongs to the tissue kallikrein family.
• Its primary physiological role is to hydrolyze semenogelin-1, thereby promoting the liquefaction of the seminal coagulum.
• The protein is synthesized as an inactive zymogen (pro-PSA) and undergoes proteolytic activation upon secretion.
• Alternative splicing generates multiple isoforms, as documented in the UniProt record.
• KLK3 is a glycoprotein containing disulfide bonds, typical of secreted enzymes, and has a well-characterized three-dimensional structure.
• Classified as a hydrolase, protease, and serine protease, it is included in the human reference proteome and has been extensively identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30–261, corresponding to the mature chain, and may mainly detect the active form of KLK3; consider using specific controls to distinguish pro- and mature forms.
• For WB, the predicted unglycosylated molecular weight is approximately 29 kDa; however, glycosylated variants can result in higher molecular weight bands.
• In IHC-P and IF/ICC, optimize antigen retrieval and permeabilization steps, as the target is a secreted protein.
• For immune-based assays such as IP and ELISA, validate the antibody in the relevant biological matrix (e.g., serum, seminal fluid) to ensure specificity and minimize interference.
• Observed cross-reactivity with mouse supports potential use in murine models, but verify sequence alignment for the target region prior to application.
• As a polyclonal antibody, confirm lot-to-lot consistency when transitioning between production batches, especially for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a seamless sourcing experience for IVD raw materials, offering validated antibodies, matched pairs, bulk ancillary reagents, and custom rare-target procurement to support diagnostic kit development. The KLK3 Rabbit pAb empowers prostate cancer biomarker research and assay optimization with reliable performance. From concept to clinic, CamelBio’s integrated supply chain streamlines your development workflow and accelerates time-to-market.