Anti-KLK15 Rabbit Polyclonal Antibody for WB, ELISA - Q9H2R5

Core Product Specifications and Parameters

Biological Background: Kallikrein-15 Function and Localization

• Kallikrein-15 (KLK15), also known as ACO protease, is a member of the tissue kallikrein family of serine proteases.
• The physiological substrate of KLK15 remains unknown; it is classified as a hydrolase with predicted serine protease activity.
• KLK15 is encoded by the KLK15 gene and is expressed as a secreted zymogen that likely requires proteolytic processing for activation.
• Highest expression levels are found in the thyroid gland, with detectable expression also in the prostate, salivary glands, adrenal glands, colon, testis, and kidney.
• The protein contains disulfide bonds and undergoes glycosylation, features common to secreted proteases.
• Alternative splicing generates multiple isoforms, and the reference proteome entry lists a molecular weight of approximately 28 kDa.
• UniProtKB keywords include: Alternative splicing, Disulfide bond, Glycoprotein, Hydrolase, Protease, Secreted, Serine protease, and Zymogen.

Experimental Guidance and Technical Tips

• The immunogen, a recombinant fragment corresponding to amino acids 80-180, was designed to target a region unique to human KLK15; consider this when assessing detection specificity.
• For Western blot, validate antibody performance under reducing conditions using lysates from tissues with known KLK15 expression, such as thyroid or prostate.
• Cross-reactivity with mouse and rat orthologs is reported; verify and optimize for each species using appropriate positive and negative controls.
• ELISA conditions (coating antigen concentration, antibody dilution, and blocking) should be empirically determined, as no standardized protocol is supplied.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with streamlined access to quality IVD raw materials. This anti-KLK15 antibody aligns with our portfolio of validated polyclonal antibodies for protease targets. From bulk ancillary reagents to rare target sourcing, we bridge the gap between discovery and clinical application.