Anti-KLK11 Polyclonal Antibody for WB, IHC-P, ELISA - Q9UBX7

Core Product Specifications and Parameters

Biological Background: KLK11 Function and Localization

• Kallikrein-11 (KLK11), also known as hippostasin, serine protease 20, or trypsin-like protease, is a secreted serine protease encoded by the KLK11 gene in the tissue kallikrein family.
• It functions as a multifunctional protease, efficiently cleaving kallikrein substrates and weakly cleaving trypsin substrates; it hydrolyzes synthetic peptides after arginine but not lysine residues.
• The protein localizes primarily to the secretory pathway, being secreted extracellularly and also detected in the Golgi apparatus.
• Tissue expression includes brain, skin, and prostate, with isoform 1 preferentially expressed in brain and isoform 2 in prostate; it is present in seminal plasma at concentrations of 2–37 µg/mL.
• Post-translational modifications include glycosylation and disulfide bond formation, and it is synthesized as an inactive zymogen requiring proteolytic activation.
• KLK11 is associated with disease variants, including ichthyosis, and may serve as a biomarker in prostate cancer and other pathologies due to its dysregulation.
• Multiple isoforms are generated by alternative splicing, potentially altering substrate specificity and tissue distribution.

Experimental Guidance and Technical Tips

• The immunogen covers the N‑terminal region (amino acids 1‑250) of human KLK11, which includes the signal peptide and propeptide domain; this design may detect both pro‑ and mature forms.
• For Western blot, consider using reducing and denaturing conditions; the predicted molecular weight is ~31 kDa, but glycosylation may result in a higher apparent mass.
• In immunohistochemistry (IHC‑P), validate antibody specificity using tissue sections with known KLK11 expression (e.g., prostate or brain) and include appropriate negative controls.
• For ELISA applications, the antibody may be used to capture or detect KLK11 in biological fluids such as seminal plasma; cross‑reactivity with mouse KLK11 enables preclinical model studies.
• Given the polyclonal nature, lot‑to‑lot consistency should be verified when used in quantitative assays; optimal working dilutions must be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high‑quality IVD raw materials like this anti‑KLK11 polyclonal antibody. As a specialized supplier, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare‑target proteins to accelerate your assay development pipeline. Whether you need single antibodies or integrated technical consulting from concept to clinic, CamelBio streamlines procurement so you can focus on advancing KLK11‑related research and clinical diagnostics.