Anti-KLHL8 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9P2G9

Core Product Specifications and Parameters

Biological Background: KLHL8 Function and Localization

• KLHL8 (Kelch-like protein 8) belongs to the KLHL family of BTB/POZ domain-containing proteins, characterized by an N-terminal BTB domain and C-terminal Kelch repeats.
• It functions as a substrate-specific adapter for the BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex, facilitating the ubiquitination and subsequent proteasomal degradation of target proteins.
• Specifically, the BCR(KLHL8) complex is required for the ubiquitination and degradation of RAPSN (43 kDa receptor-associated protein of the synapse), a protein involved in neuromuscular junction formation.
• The protein undergoes alternative splicing and post-translational acetylation, which may regulate its activity or interactions.
• Through its role in ubiquitin conjugation, KLHL8 participates in protein quality control and turnover pathways.
• KLHL8 has been identified in proteomic studies, supporting its expression in human cells.

Experimental Guidance and Technical Tips

• For western blotting, consider using this antibody to detect KLHL8 in fresh or UV-crosslinked lysates from human cell lines such as HeLa or HEK293, expecting a band near 69 kDa.
• In immunofluorescence, permeabilization with 0.1% Triton X-100 is recommended to access intracellular KLHL8; validate conditions in your target cell type.
• The antibody cross-reacts with mouse and rat, making it suitable for cross-species studies; however, sequence alignment is recommended for non-human applications.
• As a polyclonal antibody, it may recognize multiple epitopes; pre-adsorption with the immunogen or testing in knockout cell lines can help confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates your diagnostic and research programs by providing a comprehensive portfolio of IVD raw materials. For KLHL8 and related E3 ubiquitin ligase targets, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our team also specializes in sourcing rare-target raw materials, supporting your workflow from concept to clinic.