Anti-KLHL11 Rabbit Polyclonal Antibody for WB and ELISA - Q9NVR0

Core Product Specifications and Parameters

Biological Background: KLHL11 Function and Localization

• KLHL11 (Kelch-like protein 11) is a member of the kelch repeat-containing protein family.
• It functions as a substrate adaptor in a Cullin-3 (CUL3)-based E3 ubiquitin ligase complex, mediating the ubiquitination and subsequent proteasomal degradation of target proteins.
• Contains BTB (Bric-a-brac, Tramtrack, Broad Complex) and kelch repeat domains, which facilitate substrate recognition and complex assembly.
• Predicted to possess a signal peptide, suggesting possible secretion or membrane association (based on UniProt annotation).
• The protein is phosphorylated, indicating regulation by post-translational modification.
• Experimental three-dimensional structures are available (3D-structure keyword).
• Involved in the ubiquitin conjugation pathway (Ubl conjugation pathway keyword).

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 16–300 of human KLHL11; this region likely includes functional domains important for substrate binding.
• For Western blotting, a band at approximately 80 kDa corresponding to full-length KLHL11 is expected. Validate specificity using knockdown or knockout cell lysates.
• The antibody is reported to cross-react with mouse and rat KLHL11; verify performance in your specific sample system.
• For ELISA, consider using the recombinant immunogen as a positive control to confirm antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop supplier of IVD raw materials, offering validated antibodies and technical support to accelerate diagnostic development. This anti-KLHL11 polyclonal antibody is part of our extensive catalog optimized for ubiquitination research and related assay development. We also provide customized sourcing for rare targets and bulk ancillary reagents to meet your project needs.