Anti-KLHL1 Polyclonal Antibody for WB, ELISA - Q9NR64

Core Product Specifications and Parameters

Biological Background: Kelch-like protein 1 Function and Localization

• Kelch-like protein 1 (KLHL1) belongs to the kelch repeat family and contains an N-terminal BTB/POZ domain and C-terminal kelch repeats, characteristic of actin-binding proteins.
• KLHL1 is proposed to organize the actin cytoskeleton in neuronal cells, facilitating dynamic remodeling required for brain function.
• The protein exhibits cytoplasmic localization with association to the cytoskeleton, consistent with its role in actin dynamics.
• Expression of KLHL1 is highly enriched in the brain, underscoring its specialized function in the central nervous system.
• Based on its domain architecture and annotations, KLHL1 likely participates in protein-protein interactions through its kelch repeats and may act as an adaptor in signaling or cytoskeletal complexes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–210) of human KLHL1, which includes part of the BTB/POZ domain; this may confer target specificity across isoforms.
• For Western blot, consider using reduced denaturing conditions; a band near 83 kDa is expected for full-length KLHL1.
• In ELISA, validate antibody performance against recombinant or endogenous KLHL1 in the relevant sample system.
• Cross-reactivity is confirmed with human species; for other species, homology analysis is recommended before use.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated supplier of IVD raw materials, CamelBio bridges the gap from concept to clinic, offering one-stop access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For cytoskeletal and neurology targets like KLHL1, we support diagnostic manufacturers and research labs with reliable sourcing of rare-target raw materials and technical consultation to streamline assay development.