Anti-KLHDC3 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BQ90

Core Product Specifications and Parameters

Biological Background: KLHDC3 Function and Localization

• KLHDC3, also known as testis intracellular mediator protein (PEAS), is a Kelch domain-containing protein encoded by the KLHDC3 gene.
• It functions as a substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, part of the DesCEND pathway that targets proteins bearing a C-terminal glycine degron for ubiquitination and proteasomal degradation. Related references: PMID:29775578, PMID:29779948
• The CRL2(KLHDC3) complex specifically recognizes C-terminal motifs -Arg-(Xaa)n-Arg-Gly, -Arg-(Xaa)n-Lys-Gly, and -Arg-(Xaa)n-Gln-Gly. Related references: PMID:29775578, PMID:29779948
• KLHDC3 mediates the ubiquitination and degradation of truncated selenoproteins (such as SELENOV and SEPHS2) that are generated by failed UGA/Sec decoding and terminate with a glycine. Related references: PMID:26138980
• It has been implicated in the meiotic recombination process. Related references: PMID:12606021
• KLHDC3 localizes to the cytoplasm.
• The protein contains Kelch repeat motifs and is part of the ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human KLHDC3 protein (aa 1-382), which may provide broad epitope coverage for detection in Western blot and ELISA.
• For Western blot, consider using a dilution range typical for polyclonal antibodies and validate in the relevant sample system (human, mouse, rat) based on the predicted molecular weight of ~43 kDa.
• In IF/ICC, confirm subcellular localization in the cytoplasm to ensure specificity; include appropriate controls.
• For ELISA, test against recombinant protein and native samples to assess sensitivity and specificity.
• Cross-reactivity with mouse and rat suggests conserved epitopes; verify in these species with appropriate loading controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This KLHDC3 antibody supports research into ubiquitin-mediated proteolysis and cell cycle regulation. For rare target raw-material sourcing or custom development, contact our team to streamline your workflow.