Anti-KLHDC2 Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y2U9

Core Product Specifications and Parameters

Biological Background: KLHDC2 Function and Localization

• Encodes Kelch domain-containing protein 2 (KLHDC2), also designated hepatocellular carcinoma-associated antigen 33 (HCA33), host cell factor homolog LCP, and host cell factor-like protein 1 (HCLP-1).
• Serves as a substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex within the DesCEND pathway, directing ubiquitination and degradation of proteins bearing specific C-terminal degrons. Related references: PMID:29775578 PMID:29779948 PMID:30526872
• Specifically recognizes substrates ending with a diglycine (Gly-Gly) motif at the extreme C-terminus, marking them for proteasomal degradation. Related references: PMID:29775578 PMID:29779948 PMID:30526872
• Mediates the quality-control degradation of truncated selenoproteins SELENOK and SELENOS, which arise from failed UGA/Sec decoding and terminate in a Gly-Gly sequence. Related references: PMID:26138980 PMID:30526872 PMID:36805027
• Degrades proteolytically generated protein fragments ending with Gly-Gly, such as the N-terminal fragment of USP1. Related references: PMID:29775578 PMID:30526872 PMID:36805027
• Exhibits potential indirect repressor activity on CREB3-mediated transcription by disrupting CREB3-DNA binding. Related references: PMID:11384994
• Localizes predominantly to the nucleus.
• Widely expressed across tissues, with highest levels in skeletal muscle, heart, pancreas, and liver; absent in peripheral blood leukocytes. Related references: PMID:11384994 PMID:16964437

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 306–406) of human KLHDC2, which includes part of the substrate-recognition domain; this design aims to capture full-length and major isoforms.
• For western blot, the predicted molecular mass is approximately 46 kDa; confirm in your experimental system using positive controls (e.g., HeLa, HepG2 lysates) and appropriate loading controls.
• In immunohistochemistry (IHC-P), the antibody is reported to react with human and mouse tissues; consider performing antigen retrieval and titration experiments in target tissues such as liver or skeletal muscle, where KLHDC2 is highly expressed.
• For ELISA, the immunogen-based recombinant protein may serve as a positive control; optimize coating and detection conditions for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one‑stop access to IVD raw materials and technical services, accelerating development from concept to clinic. This KLHDC2 antibody supports research into ubiquitin‑mediated proteolysis and hepatocellular carcinoma biology. Through our integrated supply network, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing to streamline your assay workflows.