Anti-KLF9 Rabbit Polyclonal Antibody for WB, ELISA - Q13886

Core Product Specifications and Parameters

Biological Background: KLF9 Function and Localization

• KLF9, also known as basic transcription element-binding protein 1 (BTEB1) or GC-box-binding protein 1, is a Krueppel-like factor transcription factor encoded by the KLF9 gene.
• KLF9 binds to GC box promoter elements, selectively activating mRNA synthesis from genes with tandem GC box repeats while repressing genes with a single GC box.
• Functions as an epidermal circadian transcription factor that regulates keratinocyte proliferation, linking the circadian clock to skin homeostasis. Related references: PMID:22711835
• Predominantly localizes to the nucleus where it exerts its transcriptional regulatory functions.
• Highly expressed in the epidermis at the protein level, consistent with its role in keratinocyte biology.
• Contains zinc-finger domains that mediate DNA binding and is subject to phosphorylation, indicating potential regulation by signaling pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-150) of human KLF9; this design may enable detection of the full-length protein. Consider using this antibody for Western blot detection in relevant sample types, such as epidermal lysates from human, mouse, or rat tissues.
• As KLF9 is a nuclear transcription factor, enriching nuclear fractions could potentially improve Western blot signal specificity.
• The predicted molecular weight of KLF9 is 27 kDa, though post-translational modifications like phosphorylation may cause shifts in band migration. Validate with appropriate positive controls.
• For ELISA applications, consider using recombinant KLF9 protein as a standard and optimize assay conditions for your specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials and technical support, covering every stage from concept to clinic. This anti-KLF9 polyclonal antibody supports research into circadian rhythm regulation and transcription factor biology, and CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets to accelerate your assay development. Contact our team to explore how we can support your next project.