Anti-KLF8 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O95600

Core Product Specifications and Parameters

Biological Background: KLF8 Function and Localization

• KLF8 (Krueppel-like factor 8), also known as BKLF3 or ZNF741, is a zinc finger transcription factor belonging to the KLF family.
• Functions as both a transcriptional repressor and activator, binding to CACCC-box promoter elements and the GT-box of the cyclin D1 promoter.
• Mediates cell cycle progression at G1 phase as a downstream target of focal adhesion kinase (FAK), linking cell adhesion to proliferation.
• Localizes to the nucleus where it regulates gene expression.
• Expressed ubiquitously across tissues, suggesting a fundamental role in cellular processes.
• Contains C2H2-type zinc finger domains for DNA binding and is subject to post-translational modification including ubiquitination and isopeptide bond formation.
• Alternative splicing generates multiple transcript variants, potentially expanding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region between amino acids 210-280 of human KLF8; verify epitope conservation for your target species.
• For Western blot, a band near 39 kDa is expected; validate with appropriate positive and negative controls.
• For IF/ICC, optimize fixation and permeabilization conditions; consider using nuclei-specific counterstains to confirm nuclear localization.
• ELISA applications can be used for quantitative detection; perform standard curves and blank controls.
• Since this polyclonal antibody recognizes human, mouse, and rat KLF8, validate cross-reactivity in the relevant sample system before experimental use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop platform for IVD raw materials, technical services, and consulting—from concept to clinic. For KLF8-related research and assay development, we supply validated rabbit polyclonal antibodies, and we can source additional antibodies, recombinant proteins, and ancillary reagents to support transcription regulation and cell cycle studies. Our streamlined procurement process ensures quality and consistency for your critical projects.