Anti-KLF5 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q13887

Core Product Specifications and Parameters

Biological Background: KLF5 Function and Localization

• KLF5 (Krueppel-like factor 5) is a zinc-finger transcription factor, also known as basic transcription element-binding protein 2 (BTEB2), colon krueppel-like factor (CKLF), and intestinal-enriched krueppel-like factor (IKLF).
• It binds to GC box promoter elements and activates the transcription of target genes, functioning as a transcriptional activator.
• KLF5 is a member of the Krueppel-like factor family, which plays key roles in cell proliferation, differentiation, and embryonic development.
• Subcellular localization is predominantly nuclear, consistent with its role in regulating gene expression.
• Tissue specificity is restricted to testis and placenta, suggesting specialized functions in these reproductive and developmental tissues.
• The protein contains multiple zinc-finger domains essential for DNA binding and transcriptional regulation, and its activity may be modulated by post-translational modifications such as ubiquitination and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning the N-terminal region (amino acids 1-250) of human KLF5. This design may help target the N-terminal domain, which is less conserved among KLF family members, potentially improving specificity.
• For western blot (WB), the predicted molecular weight is 51 kDa. However, due to potential post-translational modifications or isoform variations, the observed band may differ; validate in the relevant sample system.
• This antibody is suitable for WB, IHC-P, and ELISA applications based on supplier specifications. For IHC-P, consider optimizing antigen retrieval conditions for formalin-fixed paraffin-embedded testis or placenta tissues, where KLF5 expression is reported.
• Cross-reactivity is reported for human and mouse, suggesting conserved epitopes in the N-terminal region. For other species, verify sequence homology before use.
• As a polyclonal antibody, lot-to-lot variability may occur. Include appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated B2B provider of IVD raw materials, supporting diagnostic manufacturers, research laboratories, and institutes with a comprehensive portfolio from early-stage development to clinical application. For transcription factor research involving KLF5, we offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Whatever your diagnostic or research project requires, CamelBio streamlines procurement to help you move from concept to clinic efficiently.