Anti-KLF4 Rabbit Polyclonal Antibody for WB, ELISA - O43474

Core Product Specifications and Parameters

Biological Background: KLF4 Function and Localization

• KLF4 (Krueppel-like factor 4), also known as Epithelial zinc finger protein EZF or Gut-enriched krueppel-like factor, is a transcription factor that can function as both an activator and repressor. It binds to the 5'-CACCC-3' core DNA sequence.
• Plays a critical role in regulating the expression of key transcription factors during embryonic development and is essential for maintaining embryonic stem cell pluripotency while preventing differentiation.
• Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface; involved in differentiation of epithelial cells and may also function in skeletal and kidney development.
• Contributes to the down-regulation of p53/TP53 transcription, linking it to cell cycle control and apoptosis pathways.
• Subcellularly localizes to both the nucleus and cytoplasm, consistent with its transcriptional regulatory functions and possible extra-nuclear roles.
• Contains zinc-finger DNA-binding domains, enabling direct interaction with DNA, and is subject to post-translational modifications including phosphorylation, ubiquitination, and alternative splicing, as suggested by proteomics data and structural studies.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 180-479, which includes the C-terminal zinc-finger domains critical for DNA binding. This design may help detect both full-length and truncated isoforms if present.
• For Western blot, consider using a denaturing gel with appropriate protein loading controls and validate the observed band (~55 kDa) against known KLF4 expression in human cell lines or tissues.
• In ELISA, titrate the antibody against the recombinant immunogen or cell lysates to establish optimal working concentrations, as polyclonal preparations can vary between lots.
• Given the antibody's reactivity is listed for human, and KLF4 is widely expressed in epithelial cells, consider using human skin or intestinal epithelial cell lysates as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers investigating KLF4 in transcription regulation, stem cell biology, or epithelial differentiation, we offer robust sourcing solutions for this target and related rare targets. Our comprehensive supply chain accelerates your projects from concept to clinic with reliable, research-grade materials.