Anti-KLF4 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - O43474

Core Product Specifications and Parameters

Biological Background: KLF4 Function and Localization

• KLF4 (Krueppel-like factor 4), also known as epithelial zinc finger protein EZF or gut-enriched krueppel-like factor (GKLF), is a zinc-finger transcription factor encoded by the KLF4 gene.
• It functions as both a transcriptional activator and repressor, binding to the 5'-CACCC-3' core sequence and regulating its own promoter to auto-regulate expression.
• KLF4 is essential for maintaining embryonic stem cell pluripotency and preventing differentiation, and it governs epithelial differentiation, contributing to skin barrier establishment and ocular surface maturation.
• The protein also plays roles in skeletal and kidney development and contributes to the down-regulation of p53/TP53 transcription.
• KLF4 localizes primarily to the nucleus but can be detected in the cytoplasm, reflecting dynamic regulation.
• Post-translational modifications include phosphorylation, ubiquitination (isopeptide bond), and metal-binding via zinc-finger domains, underscoring its regulatory complexity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide; thus, the antibody likely targets a linear epitope. Epitope mapping may be considered for detailed characterization.
• Validated for multiple applications including WB, IP, IF/ICC, IHC-P, and ELISA; for IHC-P, ensure appropriate antigen retrieval given the nuclear localization of KLF4.
• Cross-reactivity with mouse and rat enables use in common model organisms; validate expression in the specific tissue or cell lines of interest.
• As a rabbit monoclonal antibody, it offers batch-to-batch consistency; however, always titrate to determine optimal working concentration for each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, offering validated antibodies like this anti-KLF4 monoclonal antibody to support transcription factor and stem cell research. Beyond individual reagents, we supply antibody pairs, bulk ancillary reagents, and provide rare target sourcing for diagnostic manufacturers and laboratories. Partner with us to streamline your development from concept to clinic.