Anti-KLF15 Rabbit Polyclonal Antibody for IF/ICC and ELISA - Q9UIH9

Core Product Specifications and Parameters

Biological Background: KLF15 Function and Localization

• KLF15 (Krueppel-like factor 15), also known as kidney-enriched krueppel-like factor (KKLF), is a zinc-finger transcription factor that binds to the GA element of gene promoters.
• Acts as a transcriptional repressor of CCN2 (connective tissue growth factor) expression, thereby playing a critical role in the control of cardiac fibrosis.
• Inhibits cardiac hypertrophy by repressing the activity of key pro-hypertrophic transcription factors MEF2A and GATA4.
• Regulates circadian expression of KCNIP2 in the heart by direct promoter binding, contributing to cardiac electrophysiology.
• Involved in podocyte differentiation, indicating a role in kidney development and function.
• Functions as a negative regulator of TP53 acetylation and inhibits NF-kappa-B activation by repressing EP300-dependent RELA acetylation.
• Localized to the nucleus, consistent with its function as a DNA-binding transcriptional regulator.
• Highly expressed in liver, skeletal muscle, and kidney; also found in cardiomyocytes, but expression is reduced in certain cardiac and glomerular diseases.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein spanning the N‑terminus (aa 1‑260) of human KLF15. Consider using full‑length positive controls to confirm detection of endogenous protein.
• For IF/ICC, optimize fixation and permeabilization conditions to ensure nuclear staining; include appropriate isotype controls.
• In ELISA applications, the antibody may be used as a capture or detection reagent; titrate and validate in the relevant sample matrix.
• Due to the polyclonal nature, lot‑to‑lot consistency should be verified for critical quantitative assays.
• Cross‑reactivity is reported for human samples; reactivity with other species is not guaranteed and should be independently tested if needed.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research laboratories, and institutes streamlined access to high‑quality IVD raw materials for transcription‑related biomarkers like KLF15. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare‑target raw materials. Whether you are developing assays for cardiac fibrosis, kidney disease, or transcriptional regulation, we provide reliable, one‑stop support to move your projects from concept to clinic.