Anti-KLF14 Rabbit Polyclonal Antibody for WB, ELISA - Q8TD94

Core Product Specifications and Parameters

Biological Background: KLF14 Function and Localization

• KLF14 (Krueppel-like factor 14), also known as BTEB5 (Basic transcription element-binding protein 5), is a member of the Krueppel-like factor family of transcription factors.
• It contains zinc finger domains that enable DNA-binding and transcriptional regulation.
• KLF14 functions as a transcription factor, regulating gene expression through DNA-binding activity.
• The protein is localized to the nucleus, consistent with its role in transcription.
• It is characterized by zinc-binding and repeat regions, typical of zinc finger transcription factors.
• KLF14 is identified in proteomics studies and is part of the human reference proteome.
• The predicted molecular weight of KLF14 is approximately 33 kDa (UniProt: 33094 Da).

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 200-300) lies within the C-terminal region; verify that the antibody recognizes the expected band size in your sample system, as endogenous expression of KLF14 may vary across tissues.
• For Western blot, consider performing a BLAST search of the peptide sequence to assess potential cross-reactivity with other KLF family members.
• In ELISA, optimize coating concentration and antibody dilution using a checkerboard titration with your specific antigen or peptide.
• Given the predicted molecular weight of 33 kDa, ensure proper gel percentage and transfer conditions for resolution of proteins in this range.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material sourcing partner, connecting diagnostic manufacturers, labs, and research institutes with validated antibody pairs, bulk ancillary reagents, and specialized raw materials. This KLF14 polyclonal antibody supports research into transcriptional regulation and zinc finger biology, areas critical for biomarker discovery and assay development. Trust CamelBio to streamline your procurement from discovery to production, with expert consulting and global supply capabilities.