Anti-KLF13 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y2Y9

Core Product Specifications and Parameters

Biological Background: KLF13 Function and Localization

• KLF13, also known as BTEB3, RFLAT-1, and NSLP1, is a member of the Krueppel-like factor (KLF) family of zinc-finger transcription factors.
• It functions as both an activator and repressor of gene transcription. KLF13 activates GC-rich minimal promoters in erythroid lineage cells (By similarity) and induces RANTES (CCL5) expression in T-cells (PubMed:17513757). Related references: PMID:17513757
• As a repressor, KLF13 binds to the BTE site, a GC-rich DNA element, competing with the activator SP1, and additionally represses transcription by interacting with the corepressor Sin3A and HDAC1 (PubMed:11477107). Related references: PMID:11477107
• KLF13 localizes to the nucleus, consistent with its role as a DNA-binding transcription factor.
• It is a phosphoprotein and contains zinc-finger motifs that mediate DNA binding and metal coordination.
• KLF13 is ubiquitously expressed across tissues, indicating broad regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide encompassing a region between amino acids 100–200 of human KLF13. This region lies N-terminal to the zinc-finger domains and may generate antibodies that recognize the native protein in Western blotting.
• In Western blot (WB), a band migrating near 31 kDa is anticipated, consistent with the predicted molecular weight. We recommend using human, mouse, or rat whole-cell lysates as positive controls given the ubiquitous expression of KLF13.
• For ELISA applications, users should optimize coating conditions and antibody concentrations; cross-reactivity with mouse and rat KLF13 orthologs may be leveraged for multi-species studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets. Our polyclonal antibody portfolio, exemplified by this anti-KLF13 reagent, supports transcription factor research and diagnostic development across multiple species. Partner with us to streamline your raw-material procurement from concept to clinic.