Anti-KLF12 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y4X4

Core Product Specifications and Parameters

Biological Background: KLF12 Function and Localization

• KLF12, also known as transcriptional repressor AP-2rep, is a member of the Krüppel-like factor (KLF) family of zinc-finger transcription factors.
• It functions as a strong transcriptional repressor that binds to the A32 regulatory element in the promoter of the AP-2-alpha gene, downregulating its expression.
• The protein contains C-terminal C2H2-type zinc finger domains that mediate DNA binding to GC-rich sequences.
• KLF12 is localized predominantly in the nucleus, consistent with its role in transcriptional regulation.
• Post-translational modifications reported include phosphorylation and methylation, which may modulate its activity.
• Alternative splicing produces multiple transcript variants, potentially yielding protein isoforms with distinct regulatory properties.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 220–310, a region that may include portions of the central repressor domain or flanking sequences, likely conserved across human, mouse, and rat. Validate specificity by knocking down or overexpressing the target in your model system.
• For Western blot (WB), consider using whole-cell lysates from human, mouse, or rat cell lines; a band at ~44 kDa is expected. Optimize antibody dilution in a pilot experiment.
• For immunohistochemistry (IHC-P), nuclear staining should be observed; include appropriate positive and negative tissue controls.
• For ELISA, the antibody can be used as a capture or detection reagent; pair it with a validated matched antibody set for optimal performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with streamlined access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-KLF12, and bulk ancillary reagents for gene regulation studies. Our sourcing platform supports your entire workflow, from early-stage target evaluation to clinical assay development, offering one-stop procurement for rare-target raw materials and customized technical consulting.