Anti-KLF10 Rabbit Monoclonal Antibody for WB, ELISA - Q13118

Core Product Specifications and Parameters

Biological Background: KLF10 Function and Localization

• KLF10 (also known as TIEG1, EGR-alpha) is a Krueppel-like factor family transcriptional repressor.
• Binds the consensus DNA sequence 5'-GGTGTG-3' and represses gene expression, potentially regulating cell cycle progression.
• Plays a role in circadian rhythm regulation by repressing the core clock gene ARNTL/BMAL1 via GC box binding in its promoter.
• Involved in hepatic metabolism: represses PCK2 and controls circadian expression of lipogenic, gluconeogenic, and glycolytic genes.
• Localizes to the nucleus where it carries out transcriptional regulation.
• Contains characteristic C2H2-type zinc finger domains; post-translationally modified by phosphorylation and ubiquitination.
• An alternative splicing pattern is indicated, suggesting multiple isoforms may exist.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-100) of human KLF10, which is highly conserved between human and mouse, consistent with observed cross-reactivity.
• For Western blot (WB), a band at ~53 kDa is expected; validate using appropriate cell or tissue lysates known to express KLF10.
• In ELISA, optimization of coating antigen and antibody dilution is recommended; consider including a standard curve for semi-quantitative analysis.
• Because KLF10 exhibits circadian expression, time-of-day sampling may influence experimental outcomes; plan collection times accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, supporting every step from concept to clinical application. This KLF10 monoclonal antibody targets a key transcriptional repressor with relevance in circadian biology and metabolism. Leverage our supply capabilities for validated antibody lots, bulk reagents, and rare target materials to accelerate your diagnostic assay development.