Anti-KLF1 Rabbit Polyclonal Antibody for WB, ELISA - Q13351

Core Product Specifications and Parameters

Biological Background: KLF1 Function and Localization

• Krüppel-like factor 1 (KLF1), also known as erythroid Krüppel-like factor (EKLF), is a zinc-finger transcription factor that serves as a master regulator of erythrocyte development, acting as a general switch factor during erythropoiesis.
• KLF1 is a dual regulator of fetal-to-adult globin switching; it activates the adult beta-globin gene by binding to its CACCC box promoter element and simultaneously represses fetal gamma-globin genes (HBG1/HBG2) by upregulating the repressor BCL11A.
• KLF1 also activates the promoters of CD44 and AQP1, which contribute to erythroid membrane properties and cell signaling. Related references: PMID:21055716
• The protein belongs to the KLF family of transcription factors and contains three C-terminal C2H2-type zinc finger domains that mediate DNA binding to GC/GT-rich elements.
• KLF1 is predominantly localized in the nucleus, consistent with its role as a transcription factor.
• Expression is highly restricted to adult bone marrow and fetal liver, and is absent in myeloid and lymphoid cell lines, reflecting its erythroid-specific function.
• Post-translational modifications such as acetylation, sumoylation, phosphorylation, and methylation modulate its activity; sumoylated KLF1 acts as a repressor by recruiting the CDH2/MI2beta complex and inhibits megakaryocytic differentiation.
• Mutations in KLF1 are associated with congenital dyserythropoietic anemia type IV (CDAN4), hereditary hemolytic anemia, and the In(Lu) blood group phenotype, emphasizing its clinical importance in hematopoiesis.

Experimental Guidance and Technical Tips

• Based on the immunogen design (amino acids 211–311, which overlaps the DNA-binding domain), this antibody is expected to detect endogenous KLF1 in Western blot and ELISA applications.
• For Western blot, a band of approximately 38 kDa should be observed; we recommend using positive controls such as erythroleukemia cell lines (e.g., K562) or bone marrow lysates, as KLF1 expression is erythroid-specific.
• Cross-reactivity with mouse and rat KLF1 has been observed, but confirmatory validation in your specific experimental model is advised.
• In ELISA, consider using recombinant KLF1 protein as a standard and titrate antibody concentrations to optimize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This KLF1 polyclonal antibody supports the development of assays in hematology, genetic disease screening, and globin switching research. We offer a comprehensive portfolio including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare targets to streamline your diagnostic development pipeline.