Anti-KLB Polyclonal Antibody for WB, ELISA - Q86Z14

Core Product Specifications and Parameters

Biological Background: Beta-klotho (KLB) Function and Localization

• KLB encodes beta-klotho, a single-pass type I membrane protein that functions as a coreceptor for fibroblast growth factor receptors (FGFRs), specifically enhancing the binding of FGF21 to FGFR1 and FGFR4.
• Beta-klotho is involved in metabolic regulation by contributing to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis, thus influencing bile acid homeostasis.
• The protein is probably inactive as a glycosidase, despite structural similarities to glycoside hydrolases, indicating its primary role is non-enzymatic in nature.
• Subcellularly, beta-klotho is located at the cell membrane, consistent with its function as a transmembrane coreceptor.
• Key structural features include a signal peptide, an extracellular domain with two glycosyl hydrolase-like repeats, a single transmembrane helix, and a short cytoplasmic tail, as annotated by keywords such as Glycoprotein, Membrane, Transmembrane, and Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 720-990 within the C-terminal region of human KLB, a sequence that may map to the extracellular domain, making this antibody suitable for detecting both full-length and shed forms of beta-klotho.
• For WB applications, consider using reducing conditions and a high molecular weight marker range (around 120kDa) to resolve the glycosylated protein; note that actual observed bands may differ due to post-translational modifications.
• Due to sequence conservation, cross-reactivity with mouse and rat is supported; validate in the relevant sample system for quantitative comparisons.
• As a polyclonal antibody, it may provide higher sensitivity for ELISA; however, lot-to-lot consistency should be assessed if used in quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive procurement solution for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For researchers and diagnostic manufacturers focused on metabolic signaling and FGF21-related pathways, this anti-KLB antibody is available along with complementary products and rare target raw-material sourcing. Our streamlined supply chain bridges the gap from concept to clinic, supporting your entire development pipeline.