Anti-KITLG Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P21583

Core Product Specifications and Parameters

Biological Background: KITLG Function and Localization

• Kit ligand (KITLG), also known as stem cell factor (SCF), mast cell growth factor (MGF), and c-Kit ligand, is a crucial cytokine that serves as the ligand for the receptor tyrosine kinase KIT.
• KITLG/SCF binding to KIT activates multiple signaling cascades, including PI3K/AKT, RAS/MAPK, and STAT pathways, promoting cell survival, proliferation, and differentiation in hematopoietic, melanogenic, and germ cell lineages.
• The protein plays essential roles in stem cell maintenance, gametogenesis, mast cell development and function, and melanogenesis, acting synergistically with other interleukins.
• Subcellular localization includes the cell membrane, cytoplasm, cytoskeleton, and secreted forms; it is also found in cell projections such as lamellipodia and filopodia.
• KITLG exists in both transmembrane and soluble isoforms due to alternative splicing, with the transmembrane form capable of juxtacrine signaling.
• Post-translational modifications include glycosylation and disulfide bond formation, as indicated by keywords.
• Mutations in KITLG are associated with human diseases, including nonsyndromic deafness (DFNA69) and Waardenburg syndrome type 2F (WS2F), highlighting its role in melanocyte development.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 26-214, corresponding to the extracellular domain of human KITLG, which is well-conserved in mouse; thus, cross-reactivity is expected. Consider validating end-user-specific sample types.
• For Western blotting, the predicted molecular weight is 31 kDa, but glycosylation may result in a band around 30-40 kDa. Use appropriate controls.
• For immunofluorescence/ICC, verify fixation and permeabilization conditions to detect both membrane and secreted forms.
• In ELISA, pair with capture antibodies if developing sandwich assays, and test against recombinant KITLG standards.
• Since KITLG can exist as soluble and membrane-bound forms, consider cell treatment (e.g., serum starvation) or lineage-specific cell lines for optimal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to high-quality IVD raw materials. This anti-KITLG polyclonal antibody supports applications in hematopoiesis, oncology, and developmental biology research. Complement your projects with our validated antibody pairs, recombinant proteins, and bulk ancillary reagents, backed by expert technical support from concept to clinic.